transposition mixture (Illumina Inc)

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Illumina Inc transposition mixture
Transposition Mixture, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 990 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transposition+mix/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc13000455-121-13-22
Average 97 stars, based on 990 article reviews

transposition mixture - by Bioz Stars, 2026-10

97/100 stars

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Article Title: SOX2 phosphorylation during mitosis limits genomic damage.
Article Snippet: The nuclei were resuspended in the transposition mix (1× tagment DNA buffer [Illumina 20034197], 0.33× PBS, 0.1% Tween-20, 0.01% digitonin).

Article Title: Activity of the SWI/SNF complex is indispensable for syncytiotrophoblast formation
Article Snippet: Nuclei were then pelleted and resuspended in a transposition mix [1×TD buffer and 2.5 μl TDE1 TD enzyme (both from Illumina, 20034198), 16.5 μl PBS, 0.01% digitonin, 0.1% Tween20].

Article Title: Epigenetic profiling of hematopoietic stem cells from male mice identifies KDR and PU.1 as regulators of aging transcriptome and caloric restriction response
Article Snippet: The pellet was then resuspended in 50 μl of transposition mix (25 μl of 2x TD buffer, 2.5 μl of Tn5 Transposase (20034197, Illumina), 22.5 μl of H 2 O) by pipetting up and down 6 times.

Article Title: A Plastic EMP1 + to LGR5 + Cell State Conversion as a Bypass to KRAS G12D Pharmacologic Inhibition in Metastatic Colorectal Cancer
Article Snippet: Counting was done in RSB with a Neubauer chamber, and aliquots of 50,000 nuclei were prepared and resuspended in 50 μL transposition mix [25 μL 2x TD buffer (Illumina, 20034197), 2.5 μL transposase (Illumina, 20034197), 16.5 μL PBS, 0.5 μL 1% digitonin (Millipore, 300410), 0.5% 10% Tween-20 (Sigma-Aldrich, P9416), and 5 μL water] by pipetting up and down 10 times.

Article Title: Epigenetic profiling of hematopoietic stem cells from male mice identifies KDR and PU.1 as regulators of aging transcriptome and caloric restriction response.
Article Snippet: The pellet was then resuspended in 50 μl of transposition mix (25 μl of 2x TD buffer, 2.5 μl of Tn5 Transposase (20034197, Illumina), 22.5 μl of H2O) by pipetting up and down 6 times.

Article Title: SOX2 phosphorylation during mitosis limits genomic damage
Article Snippet: The nuclei were resuspended in the transposition mix (1× tagment DNA buffer [Illumina 20034197], 0.33× PBS, 0.1% Tween-20, 0.01% digitonin).

Incubation:

Article Title: Distinct epigenetic and metabolic states reflect the regional identity of adult neural stem cells and prime their fate
Article Snippet: .. Pellets were re-suspended in 50 μl transposition mix (#20034197, Illumina) and incubated for 30 min at 37 °C. .. Libraries were amplified by PCR with barcoded Nextera primers and sequenced.

Article Title: RIPK3-RNASE1 axis as a potential therapeutic and clinical monitoring target in VEXAS syndrome
Article Snippet: .. Subsequently, nuclei were incubated with a transposition mix (1× TD buffer, 100 nM transposase [TDE1; Cat. no. 20034198; Illumina], 33% 1× PBS [33% of the total volume of the transposition mix was 1× PBS], 0.01% digitonin, 0.1% Tween-20, and 5% water) for 30 min at 37°C with shaking at 900 rpm. .. The tagged DNA was purified using the MinElute Reaction Cleanup Kit (Cat. No. 28204; QIAGEN).

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